sheep anti erbb3 r d systems  (R&D Systems)

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: The cooperative induction of hypoxia-inducible factor-1 alpha and STAT3 during hypoxia induced an impairment of tumor susceptibility to CTL-mediated cell lysis.

doi: 10.4049/jimmunol.0800854

Figure Lengend Snippet: FIGURE 4. Involvement of VEGF in STAT3 activation under hypoxia and relationship with JAK/STAT3 pathway. A, IGR-Heu tumor cells were kept under normoxia (21% PO2) and hypoxia (1% PO2) for 24 h. ELISA was performed for quantification of IL-6, IL-10, and VEGF by Quantikine Human immunoassay R&D Systems. Bars, SD. The results shown are representative of two independent experiments. B, IGR-Heu tumor cells were incubated for 24 h with 25, 50, and 100 g/ml Avastin (Genentech) or an isotype control Ab (human IgG1; Sigma-Aldrich) in reduced serum conditions (0.5% FBS) under (21% PO2) and hypoxia (1% PO2). Whole-cell lysates (30 g) were subjected to SDS-PAGE, blotted, and probed with Abs, as indicated. Actin was used as the loading control. C, Attenuation of IGR-Heu tumor cells resistance to CTL (Heu171)-mediated lysis under hypoxic conditions following treatment with anti-VEGF (Avastin). IGR-Heu tumor cells were incubated for 24 h with 25 g/ml Avastin or an isotype control Ab (human IgG1; Sigma-Aldric h) under normoxia (21% PO2) and hypoxia (1% PO2). Cytotoxicity was determined by a conventional 4-h 51Cr release assay. Heu171 (TIL-derived T cell clone) was used as effectors. Bars, SD.

Article Snippet: A, IGR-Heu tumor cells were kept under normoxia (21% PO2) and hypoxia (1% PO2) for 24 h. ELISA was performed for quantification of IL-6, IL-10, and VEGF by Quantikine Human immunoassay R&D Systems.

Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay, Incubation, Control, SDS Page, Lysis, Release Assay, Derivative Assay